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inpp5e phosphatase domains with fkbp  (Addgene inc)


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    Addgene inc inpp5e phosphatase domains with fkbp
    a Schematic figure showing Pseudojanin (PJ), PJ-Sac, <t>PJ-INPP5E,</t> and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct. b Schematic figure showing Pseudojanin (PJ) and the membrane targeted Lyn-11-FRB-mCherry construct together with the PI4P biosensor SidM-GFP before and after rapamycin treatment. c Representative confocal images in HEK293T cells expressing PLCδ1-PH-GFP and the PJ constructs indicated (PJ, PJ-Sac, PJ-INPP5E or PJ-DEAD) before and after rapamycin treatment (1 μM, 30 min). d Summary data of PLCδ1-PH-GFP (top) and SidM-GFP (bottom) membrane to cytoplasmic fluorescence ratio (M/C) measured. Cells were treated as indicated. Scale bars, 5 μM. * p < 0.05. number of cells indicated in parenthesis.* p < 0.05.
    Inpp5e Phosphatase Domains With Fkbp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inpp5e phosphatase domains with fkbp/product/Addgene inc
    Average 93 stars, based on 18 article reviews
    inpp5e phosphatase domains with fkbp - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Membrane pools of phosphatidylinositol-4-phosphate regulate KCNQ1/KCNE1 membrane expression"

    Article Title: Membrane pools of phosphatidylinositol-4-phosphate regulate KCNQ1/KCNE1 membrane expression

    Journal: Communications Biology

    doi: 10.1038/s42003-021-02909-1

    a Schematic figure showing Pseudojanin (PJ), PJ-Sac, PJ-INPP5E, and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct. b Schematic figure showing Pseudojanin (PJ) and the membrane targeted Lyn-11-FRB-mCherry construct together with the PI4P biosensor SidM-GFP before and after rapamycin treatment. c Representative confocal images in HEK293T cells expressing PLCδ1-PH-GFP and the PJ constructs indicated (PJ, PJ-Sac, PJ-INPP5E or PJ-DEAD) before and after rapamycin treatment (1 μM, 30 min). d Summary data of PLCδ1-PH-GFP (top) and SidM-GFP (bottom) membrane to cytoplasmic fluorescence ratio (M/C) measured. Cells were treated as indicated. Scale bars, 5 μM. * p < 0.05. number of cells indicated in parenthesis.* p < 0.05.
    Figure Legend Snippet: a Schematic figure showing Pseudojanin (PJ), PJ-Sac, PJ-INPP5E, and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct. b Schematic figure showing Pseudojanin (PJ) and the membrane targeted Lyn-11-FRB-mCherry construct together with the PI4P biosensor SidM-GFP before and after rapamycin treatment. c Representative confocal images in HEK293T cells expressing PLCδ1-PH-GFP and the PJ constructs indicated (PJ, PJ-Sac, PJ-INPP5E or PJ-DEAD) before and after rapamycin treatment (1 μM, 30 min). d Summary data of PLCδ1-PH-GFP (top) and SidM-GFP (bottom) membrane to cytoplasmic fluorescence ratio (M/C) measured. Cells were treated as indicated. Scale bars, 5 μM. * p < 0.05. number of cells indicated in parenthesis.* p < 0.05.

    Techniques Used: Construct, Membrane, Expressing, Fluorescence

    a Schematic figure showing Pseudojanin (PJ), PJ-Sac, PJ-INPP5E, and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct together with the channel before and after rapamycin treatment. b Left: representative HEK293T cells expressing KCNQ1-GFP, KCNE1, and the PJ constructs indicated after rapamycin treatment (1 μM, 30 min). Right: summary data of membrane to cytoplasmic fluorescence ratio (M/C) of KCNQ1-GFP measured in experiments as the left panel. Cells were treated as indicated. Scale bars, 5 µM. * p < 0.05, number of cells at least 27.
    Figure Legend Snippet: a Schematic figure showing Pseudojanin (PJ), PJ-Sac, PJ-INPP5E, and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct together with the channel before and after rapamycin treatment. b Left: representative HEK293T cells expressing KCNQ1-GFP, KCNE1, and the PJ constructs indicated after rapamycin treatment (1 μM, 30 min). Right: summary data of membrane to cytoplasmic fluorescence ratio (M/C) of KCNQ1-GFP measured in experiments as the left panel. Cells were treated as indicated. Scale bars, 5 µM. * p < 0.05, number of cells at least 27.

    Techniques Used: Construct, Membrane, Expressing, Fluorescence



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    inpp5e phosphatase domains with fkbp  (Addgene inc)
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    a Schematic figure showing Pseudojanin (PJ), PJ-Sac, <t>PJ-INPP5E,</t> and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct. b Schematic figure showing Pseudojanin (PJ) and the membrane targeted Lyn-11-FRB-mCherry construct together with the PI4P biosensor SidM-GFP before and after rapamycin treatment. c Representative confocal images in HEK293T cells expressing PLCδ1-PH-GFP and the PJ constructs indicated (PJ, PJ-Sac, PJ-INPP5E or PJ-DEAD) before and after rapamycin treatment (1 μM, 30 min). d Summary data of PLCδ1-PH-GFP (top) and SidM-GFP (bottom) membrane to cytoplasmic fluorescence ratio (M/C) measured. Cells were treated as indicated. Scale bars, 5 μM. * p < 0.05. number of cells indicated in parenthesis.* p < 0.05.
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    https://www.bioz.com/result/inpp5e phosphatase domains with fkbp/product/Addgene inc
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    inpp5e domain  (Addgene inc)
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    a Schematic figure showing Pseudojanin (PJ), PJ-Sac, <t>PJ-INPP5E,</t> and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct. b Schematic figure showing Pseudojanin (PJ) and the membrane targeted Lyn-11-FRB-mCherry construct together with the PI4P biosensor SidM-GFP before and after rapamycin treatment. c Representative confocal images in HEK293T cells expressing PLCδ1-PH-GFP and the PJ constructs indicated (PJ, PJ-Sac, PJ-INPP5E or PJ-DEAD) before and after rapamycin treatment (1 μM, 30 min). d Summary data of PLCδ1-PH-GFP (top) and SidM-GFP (bottom) membrane to cytoplasmic fluorescence ratio (M/C) measured. Cells were treated as indicated. Scale bars, 5 μM. * p < 0.05. number of cells indicated in parenthesis.* p < 0.05.
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    sac1 domain  (Addgene inc)
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    a Schematic figure showing Pseudojanin (PJ), PJ-Sac, <t>PJ-INPP5E,</t> and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct. b Schematic figure showing Pseudojanin (PJ) and the membrane targeted Lyn-11-FRB-mCherry construct together with the PI4P biosensor SidM-GFP before and after rapamycin treatment. c Representative confocal images in HEK293T cells expressing PLCδ1-PH-GFP and the PJ constructs indicated (PJ, PJ-Sac, PJ-INPP5E or PJ-DEAD) before and after rapamycin treatment (1 μM, 30 min). d Summary data of PLCδ1-PH-GFP (top) and SidM-GFP (bottom) membrane to cytoplasmic fluorescence ratio (M/C) measured. Cells were treated as indicated. Scale bars, 5 μM. * p < 0.05. number of cells indicated in parenthesis.* p < 0.05.
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    Image Search Results


    a Schematic figure showing Pseudojanin (PJ), PJ-Sac, PJ-INPP5E, and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct. b Schematic figure showing Pseudojanin (PJ) and the membrane targeted Lyn-11-FRB-mCherry construct together with the PI4P biosensor SidM-GFP before and after rapamycin treatment. c Representative confocal images in HEK293T cells expressing PLCδ1-PH-GFP and the PJ constructs indicated (PJ, PJ-Sac, PJ-INPP5E or PJ-DEAD) before and after rapamycin treatment (1 μM, 30 min). d Summary data of PLCδ1-PH-GFP (top) and SidM-GFP (bottom) membrane to cytoplasmic fluorescence ratio (M/C) measured. Cells were treated as indicated. Scale bars, 5 μM. * p < 0.05. number of cells indicated in parenthesis.* p < 0.05.

    Journal: Communications Biology

    Article Title: Membrane pools of phosphatidylinositol-4-phosphate regulate KCNQ1/KCNE1 membrane expression

    doi: 10.1038/s42003-021-02909-1

    Figure Lengend Snippet: a Schematic figure showing Pseudojanin (PJ), PJ-Sac, PJ-INPP5E, and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct. b Schematic figure showing Pseudojanin (PJ) and the membrane targeted Lyn-11-FRB-mCherry construct together with the PI4P biosensor SidM-GFP before and after rapamycin treatment. c Representative confocal images in HEK293T cells expressing PLCδ1-PH-GFP and the PJ constructs indicated (PJ, PJ-Sac, PJ-INPP5E or PJ-DEAD) before and after rapamycin treatment (1 μM, 30 min). d Summary data of PLCδ1-PH-GFP (top) and SidM-GFP (bottom) membrane to cytoplasmic fluorescence ratio (M/C) measured. Cells were treated as indicated. Scale bars, 5 μM. * p < 0.05. number of cells indicated in parenthesis.* p < 0.05.

    Article Snippet: Additional constructs used were Pseudojanin (PJ), a fusion protein of Sac and INPP5E phosphatase domains with FKBP (Addgene, #37999); PJ-Sac, a PJ based construct containing an inactivating mutation (Asp1263Ala) in the INPP5E domain (Addgene, #38000); PJ-INPP5E, a PJ based construct with an inactivating mutation (Cys779Ser) in the SAC1 domain (Addgene, #38001); PJ-DEAD, a PJ based construct containing an inactivating mutation (Asp1263Ala) in the INPP5E domain and an inactivating mutation (Cys779Ser) in the SAC1 domain (Addgene, #38002) , ; PH-PLCδ1-GFP (Addgene, #51407); LYN11-FRB-mcherry (Addgene, #38004); GFP-P4M-SidM (Addgene, #51469), hKCNH2-EGFP (VectorBuilder); EGFP-Kir2.1 (Addgene, #107184); Kv7.4-EGFP (Addgene, #111453); Kv2.1-EGFP (Addgene, #111531); constitutively active PI4K (Addgene, #139311); dominant negative PI4K-dead (Addgene, #139312); KCNE1-KCNQ1 tandem .

    Techniques: Construct, Membrane, Expressing, Fluorescence

    a Schematic figure showing Pseudojanin (PJ), PJ-Sac, PJ-INPP5E, and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct together with the channel before and after rapamycin treatment. b Left: representative HEK293T cells expressing KCNQ1-GFP, KCNE1, and the PJ constructs indicated after rapamycin treatment (1 μM, 30 min). Right: summary data of membrane to cytoplasmic fluorescence ratio (M/C) of KCNQ1-GFP measured in experiments as the left panel. Cells were treated as indicated. Scale bars, 5 µM. * p < 0.05, number of cells at least 27.

    Journal: Communications Biology

    Article Title: Membrane pools of phosphatidylinositol-4-phosphate regulate KCNQ1/KCNE1 membrane expression

    doi: 10.1038/s42003-021-02909-1

    Figure Lengend Snippet: a Schematic figure showing Pseudojanin (PJ), PJ-Sac, PJ-INPP5E, and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct together with the channel before and after rapamycin treatment. b Left: representative HEK293T cells expressing KCNQ1-GFP, KCNE1, and the PJ constructs indicated after rapamycin treatment (1 μM, 30 min). Right: summary data of membrane to cytoplasmic fluorescence ratio (M/C) of KCNQ1-GFP measured in experiments as the left panel. Cells were treated as indicated. Scale bars, 5 µM. * p < 0.05, number of cells at least 27.

    Article Snippet: Additional constructs used were Pseudojanin (PJ), a fusion protein of Sac and INPP5E phosphatase domains with FKBP (Addgene, #37999); PJ-Sac, a PJ based construct containing an inactivating mutation (Asp1263Ala) in the INPP5E domain (Addgene, #38000); PJ-INPP5E, a PJ based construct with an inactivating mutation (Cys779Ser) in the SAC1 domain (Addgene, #38001); PJ-DEAD, a PJ based construct containing an inactivating mutation (Asp1263Ala) in the INPP5E domain and an inactivating mutation (Cys779Ser) in the SAC1 domain (Addgene, #38002) , ; PH-PLCδ1-GFP (Addgene, #51407); LYN11-FRB-mcherry (Addgene, #38004); GFP-P4M-SidM (Addgene, #51469), hKCNH2-EGFP (VectorBuilder); EGFP-Kir2.1 (Addgene, #107184); Kv7.4-EGFP (Addgene, #111453); Kv2.1-EGFP (Addgene, #111531); constitutively active PI4K (Addgene, #139311); dominant negative PI4K-dead (Addgene, #139312); KCNE1-KCNQ1 tandem .

    Techniques: Construct, Membrane, Expressing, Fluorescence

    a Schematic figure showing Pseudojanin (PJ), PJ-Sac, PJ-INPP5E, and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct. b Schematic figure showing Pseudojanin (PJ) and the membrane targeted Lyn-11-FRB-mCherry construct together with the PI4P biosensor SidM-GFP before and after rapamycin treatment. c Representative confocal images in HEK293T cells expressing PLCδ1-PH-GFP and the PJ constructs indicated (PJ, PJ-Sac, PJ-INPP5E or PJ-DEAD) before and after rapamycin treatment (1 μM, 30 min). d Summary data of PLCδ1-PH-GFP (top) and SidM-GFP (bottom) membrane to cytoplasmic fluorescence ratio (M/C) measured. Cells were treated as indicated. Scale bars, 5 μM. * p < 0.05. number of cells indicated in parenthesis.* p < 0.05.

    Journal: Communications Biology

    Article Title: Membrane pools of phosphatidylinositol-4-phosphate regulate KCNQ1/KCNE1 membrane expression

    doi: 10.1038/s42003-021-02909-1

    Figure Lengend Snippet: a Schematic figure showing Pseudojanin (PJ), PJ-Sac, PJ-INPP5E, and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct. b Schematic figure showing Pseudojanin (PJ) and the membrane targeted Lyn-11-FRB-mCherry construct together with the PI4P biosensor SidM-GFP before and after rapamycin treatment. c Representative confocal images in HEK293T cells expressing PLCδ1-PH-GFP and the PJ constructs indicated (PJ, PJ-Sac, PJ-INPP5E or PJ-DEAD) before and after rapamycin treatment (1 μM, 30 min). d Summary data of PLCδ1-PH-GFP (top) and SidM-GFP (bottom) membrane to cytoplasmic fluorescence ratio (M/C) measured. Cells were treated as indicated. Scale bars, 5 μM. * p < 0.05. number of cells indicated in parenthesis.* p < 0.05.

    Article Snippet: Additional constructs used were Pseudojanin (PJ), a fusion protein of Sac and INPP5E phosphatase domains with FKBP (Addgene, #37999); PJ-Sac, a PJ based construct containing an inactivating mutation (Asp1263Ala) in the INPP5E domain (Addgene, #38000); PJ-INPP5E, a PJ based construct with an inactivating mutation (Cys779Ser) in the SAC1 domain (Addgene, #38001); PJ-DEAD, a PJ based construct containing an inactivating mutation (Asp1263Ala) in the INPP5E domain and an inactivating mutation (Cys779Ser) in the SAC1 domain (Addgene, #38002) , ; PH-PLCδ1-GFP (Addgene, #51407); LYN11-FRB-mcherry (Addgene, #38004); GFP-P4M-SidM (Addgene, #51469), hKCNH2-EGFP (VectorBuilder); EGFP-Kir2.1 (Addgene, #107184); Kv7.4-EGFP (Addgene, #111453); Kv2.1-EGFP (Addgene, #111531); constitutively active PI4K (Addgene, #139311); dominant negative PI4K-dead (Addgene, #139312); KCNE1-KCNQ1 tandem .

    Techniques: Construct, Membrane, Expressing, Fluorescence

    a Schematic figure showing Pseudojanin (PJ), PJ-Sac, PJ-INPP5E, and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct together with the channel before and after rapamycin treatment. b Left: representative HEK293T cells expressing KCNQ1-GFP, KCNE1, and the PJ constructs indicated after rapamycin treatment (1 μM, 30 min). Right: summary data of membrane to cytoplasmic fluorescence ratio (M/C) of KCNQ1-GFP measured in experiments as the left panel. Cells were treated as indicated. Scale bars, 5 µM. * p < 0.05, number of cells at least 27.

    Journal: Communications Biology

    Article Title: Membrane pools of phosphatidylinositol-4-phosphate regulate KCNQ1/KCNE1 membrane expression

    doi: 10.1038/s42003-021-02909-1

    Figure Lengend Snippet: a Schematic figure showing Pseudojanin (PJ), PJ-Sac, PJ-INPP5E, and PJ-dead constructs and the membrane targeted Lyn-11-FRB-mCherry construct together with the channel before and after rapamycin treatment. b Left: representative HEK293T cells expressing KCNQ1-GFP, KCNE1, and the PJ constructs indicated after rapamycin treatment (1 μM, 30 min). Right: summary data of membrane to cytoplasmic fluorescence ratio (M/C) of KCNQ1-GFP measured in experiments as the left panel. Cells were treated as indicated. Scale bars, 5 µM. * p < 0.05, number of cells at least 27.

    Article Snippet: Additional constructs used were Pseudojanin (PJ), a fusion protein of Sac and INPP5E phosphatase domains with FKBP (Addgene, #37999); PJ-Sac, a PJ based construct containing an inactivating mutation (Asp1263Ala) in the INPP5E domain (Addgene, #38000); PJ-INPP5E, a PJ based construct with an inactivating mutation (Cys779Ser) in the SAC1 domain (Addgene, #38001); PJ-DEAD, a PJ based construct containing an inactivating mutation (Asp1263Ala) in the INPP5E domain and an inactivating mutation (Cys779Ser) in the SAC1 domain (Addgene, #38002) , ; PH-PLCδ1-GFP (Addgene, #51407); LYN11-FRB-mcherry (Addgene, #38004); GFP-P4M-SidM (Addgene, #51469), hKCNH2-EGFP (VectorBuilder); EGFP-Kir2.1 (Addgene, #107184); Kv7.4-EGFP (Addgene, #111453); Kv2.1-EGFP (Addgene, #111531); constitutively active PI4K (Addgene, #139311); dominant negative PI4K-dead (Addgene, #139312); KCNE1-KCNQ1 tandem .

    Techniques: Construct, Membrane, Expressing, Fluorescence